Rock River Laboratory Mold IdentificationAnti-Nutritional Measures

Manage the risks posed by bacteria, mold, yeast, and mycotoxins through count, identification, and screening options

Bacteria, mold, yeast, and mycotoxins are in a class all of their own, and by wreacking havoc on animal feeds, they've earned the category name of "anti-nutritional factors". Managing the risks associated with these organisms is possible through counts, identification, and screening as soon as there is suspect. Rock River Laboratory offers basic and advanced analysis for bacteria, mold, yeast, and mycotoxins, while also partnering with other laboratories for more involved needs. Analyses offered, along with guidelines for resulting count levels can be reviewed below. Learn more about the beginnings and effects of these anti-nutritional factors in this article from Progressive Dairyman.


Bacteria, Mold, Yeast, and Mycotoxin Analyses

  • TMR Hygiene Diagnostic package
  • Yeast and mold count: Requires a 5-day incubation
  • Yeast and mold count and identification
  • Rapid mold and yeast count: For those needing counts quickly, this analysis provides mold and yeast levels after a 2-day incubation, but does not offer identification.
  • Clostridium perfringens count
  • Total enterobacteria count: Measures bacteria count of all species included in the Enterobacteriaceae family
  • Mycotoxin analysis: DON, Zearalenone, T-2, Fumonisin, or Aflatoxin, 
  • Fusarium mycotoxin screen: Scans for 13 toxins from Fusarium
  • Basic mycotoxin screen: Scans for 12 toxins from Fusarium and Aflatoxin
  • Comprehensive mycotoxin screen: Scans for 19 toxins from Fusarium and Citrinin along with basic toxins

Guidelines for Resulting Levels of Mold, Yeast, and Toxins

Fungal and Bacterial Count Guidelines for Agricultural Feed and Total Mixed Rations

Mycotoxin Guidelines and Dietary Limits


Mycotoxin Analyses

Mycotoxins can lie unrecognized in feed and may prove detrimental to an animal’s performance. Analyzing feedstuffs is the first step to mitigating these animal health and production threats. Rock River Laboratory’s mycotoxin analysis takes testing to the next level to help shed light on the root of on-farm ration challenges by offering:

Timely results

Mycotoxin screens can be completed in as little as three to four days* – helping diagnose feedstuff challenges quickly. The faster the analysis results, the quicker a mitigation strategy can be put into action to stop production losses and improve animal health.

Accurate analysis 

Rock River Laboratory employs a mass spectrometry technique, the gold standard in animal nutrition and biochemistry, to analyze samples for mycotoxin concentrations. Mass spectrometry offers unmatched accuracy and precision, with better detection limits than other techniques.

Visual reports

Deciphering mycotoxin level reports shouldn’t require a PhD degree. Rock River Laboratory has crafted a visual, interpretive report to easily showcase where mycotoxin levels lie on the spectrum of ‘below detection limit’, to ‘concern’ level, to ‘near-maximum concentrations ever recorded’ for a total diet - allowing for quick and sound decisions. Explore our example visual mycotoxin report here.

Rock River Laboratory’s team has the mycotoxin expertise and supporting resources available to assist when such challenges arise. Contact Rock River Laboratory to learn more, and download our information sheet here.
Mycotoxin Analysis Packages

When it comes to analyzing feed and feed products for anti-nutritional substances beyond yeast and mold, Rock River Laboratory offers a variety of mycotoxin analysis packages. There is a boundless amount of information regarding which toxins, at what levels, cause certain ailments.  The focus here is to help you decide which package is right for you based on your identifying needs.

Common Mycotoxin Screen [Most Popular Mycotoxin Package]

7 mycotoxins included

Analyte

Limit of Detection

Method

Aflatoxin B1

<12 ppb

LCMSMS

Aflatoxin B2

<12 ppb

LCMSMS

Aflatoxin G1

<12 ppb

LCMSMS

Aflatoxin G2

<12 ppb

LCMSMS

Deoxynivalenol

<25 ppb

LCMSMS

T-2 Toxin

<50 ppb

LCMSMS

Zearalenone

<75 ppb

LCMSMS

Comprehensive Mycotoxin Screen

19 mycotoxins included

Analyte

Limit of Detection

Method

15-Acetyl Deoxynivalenol

0.1 ppm

LCMSMS

3-Acetyl Deoxynivalenol

0.1 ppm

LCMSMS

Aflatoxin B1

1 ppb

LCMSMS

Aflatoxin B2

1 ppb

LCMSMS

Aflatoxin G1

1 ppb

LCMSMS

Aflatoxin G2

1 ppb

LCMSMS

Citrinin

50 ppb

LCMSMS

Diacetoxyscirpenol

20ppb

LCMSMS

Deoxynivalenol

0.1 ppm

HPLC

Fumonisin B1

0.1 ppm

LCMSMS

Fumonisin B2

0.1 ppm

LCMSMS

Fumonisin B3

0.1 ppm

LCMSMS

Fusarenon X

0.1 ppm

LCMSMS

HT-2 Toxin

5 ppb

LCMSMS

Neosolaniol

20 ppb

LCMSMS

Nivalenol

0.1 ppm

LCMSMS

Ochratoxin A

1 ppb

LCMSMS

T-2 Toxin

5 ppb

LCMSMS

Zearalenone

50 ppb

HPLC


Basic Mycotoxin Screen

11 mycotoxins included

Analyte

Limit of Detection

Method

Aflatoxin B1

1 ppb

LCMSMS

Aflatoxin B2

1 ppb

LCMSMS

Aflatoxin G1

1 ppb

LCMSMS

Aflatoxin G2

1 ppb

LCMSMS

Deoxynivalenol

0.1 ppm

LCMSMS

Fumonisin B1

0.1 ppm

LCMSMS

Fumonisin B2

0.1 ppm

LCMSMS

Fumonisin B3

0.1 ppm

LCMSMS

Ochratoxin A

1 ppb

LCMSMS

T-2 Toxin

5 ppb

LCMSMS

Zearalenone

12.5 ppb

LCMSMS

Fusarium Mycotoxin Screen

13 mycotoxins produced by Fusarium

Analyte

Limit of Detection

Method

15-Acetyl Deoxynivalenol

0.1 ppb

GC

3-Acetyl Deoxynivalenol

0.1 ppb

GC

Deoxynivalenol

0.1 ppb

GC

Diacetoxyscirpenol

100 ppb

GC

Fumonisin B1

0.1 ppm

HPLC

Fumonisin B2

0.1 ppm

HPLC

Fumonisin B3

0.1 ppm

HPLC

Fusarenon X

0.1 ppm

GC

HT-2 Toxin

100 ppb

GC

Neosolaniol

100 ppb

GC

Nivalenol

0.1 ppm

GC

T-2 Toxin

100 ppb

GC

Zearalenone

50 ppb

HPLC

 

Aflatoxin Mycotoxin Screen

4 Aflatoxins identified

Analyte

Limit of Detection

Method

Aflatoxin B1

1 ppb

HPLC

Aflatoxin B2

1 ppb

HPLC

Aflatoxin G1

1 ppb

HPLC

Aflatoxin G2

1 ppb

HPLC

 

 

Rock River Laboratory

Founded in 1976, Rock River Laboratory is a family-owned laboratory network that provides production assistance to the agricultural industry through the use of advanced diagnostic systems, progressive techniques, and research-supported analyses.  Employing a team of top specialists in their respective fields, Rock River Laboratory provides accurate, cost-effective, and timely analytical results to customers worldwide, while featuring unsurpassed customer service.

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